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| Theodor Zbinden |
Thesis/Dissertation: Identification and Expression Analysis of Two
Homologs from Xenopus laevis of the Tumorhead Putative Binding Protein,
FBXO30
Tumorhead (TH) is a maternal factor that regulates cell proliferation during early embryogenesis in Xenopus laevis. To understand how TH functions at the molecular level, we have been studying its relationship with the novel F-Box containing protein FBXO30, found in a two-hybrid screen for TH binding proteins. By RT-PCR, we have found two homologs, FBXO30-A and FBXO30-B that are maternal factors as their messages are present in the unfertilized egg, which could be key regulators of early development working with TH to promote cell proliferation. To further characterize FBXO30 and its putative interaction with TH, we will study FBXO30 spatial expression throughout the embryo by in situ hybridization. We will also study FBXO30 temporal and spatial expression at the protein level performing Western Blot analysis of embryo extracts and immunostaining, respectively. In order to confirm the interaction between TH and FBXO30, we will carry out co-immunoprecipitation and co-localization experiments.
Advisor: Dr. Edwin E. Traverso
E-mail: theo.zbinden@upr.edu
Tumorhead (TH) is a maternal factor that regulates cell proliferation during early embryogenesis in Xenopus laevis. To understand how TH functions at the molecular level, we have been studying its relationship with the novel F-Box containing protein FBXO30, found in a two-hybrid screen for TH binding proteins. By RT-PCR, we have found two homologs, FBXO30-A and FBXO30-B that are maternal factors as their messages are present in the unfertilized egg, which could be key regulators of early development working with TH to promote cell proliferation. To further characterize FBXO30 and its putative interaction with TH, we will study FBXO30 spatial expression throughout the embryo by in situ hybridization. We will also study FBXO30 temporal and spatial expression at the protein level performing Western Blot analysis of embryo extracts and immunostaining, respectively. In order to confirm the interaction between TH and FBXO30, we will carry out co-immunoprecipitation and co-localization experiments.
Advisor: Dr. Edwin E. Traverso
E-mail: theo.zbinden@upr.edu
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| Rey J. Rosa Morales |
Thesis/Dissertation: Molecular aspects of intestinal regeneration.
Organogenesis and regeneration of the nervous tissue is the future. To achieve this goal, we need first to study the different events that are involved in regeneration process. We use the sea cucumber Holothuria glaberrima as a model organism because it can regenerate the gastrointestinal system after evisceration. Studying the cell-cell interaction, the extracellular matrix remodeling and the molecular signals that are being regulated during organogenesis are important for the understanding of these processes. The data obtained from these studies can contribute greatly for the future understanding of regenerative neurogenesis.
Advisor: Dr. José E. García-Arrarás
E-mail: rey.rosa@upr.edu
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